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D1283

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

With calcium chloride and magnesium chloride, 10×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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General description

Dulbecco′s Phosphate Buffered Saline is an isotonic saline solution used for washing cells and tissues. This balanced salt solution (BSS) is also used in cell culture as a buffering system to maintain the medium within the physiological pH range and as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.

Application

Dulbecco′s Phosphate Buffered Saline has been used:
  • for decellularization of extracellular matrix (ECM)
  • to rinse biosensor chip to remove physically adsorbed mycolic acids
  • for washing cells before staining during in vitro biocompatibility test

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

D1283-100ML:
D1283-BULK:
D1283-VAR:
D1283-500ML:
D1283-6X500ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biomaterials science, 5(10), 2009-2023 (2017-08-16)
Blood clots stop bleeding and provide cell-instructive microenvironments. Still, in vitro models used to study implant performance typically neglect any possible interactions of recruited cells with surface-adhering blood clots. Here we study the interaction and synergies of bone marrow derived
Janice Dias et al.
Eye & contact lens, 41(5), 281-286 (2015-01-21)
To determine the effect of hydration media on ex vivo corneal elasticity. Experiments were conducted on 40 porcine eyes retrieved from an abattoir (10 eyes each for phosphate-buffered saline (PBS), balanced salt solution, Optisol, 15% dextran). The epithelium was removed
Sarah S Gabriel et al.
Kidney international, 91(4), 842-855 (2017-02-02)
Dent disease is a rare X-linked tubulopathy caused by mutations in the endosomal chloride-proton exchanger (ClC-5) resulting in defective receptor-mediated endocytosis and severe proximal tubule dysfunction. Bone marrow transplantation has recently been shown to preserve kidney function in cystinosis, a
Nasim Shahidi Hamedani et al.
Journal of clinical medicine, 10(4) (2021-02-14)
Coagulation factor XIII (FXIII) is a protransglutaminase which plays an important role in clot stabilization and composition by cross-linking the α- and γ-chains of fibrin and increasing the resistance of the clot to mechanical and proteolytic challenges. In this study
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