BE(2)-C

95011817

• UNSPSC Code: 41106514

Properties

• biological source: human nerve
• growth mode: Adherent
• karyotype: Modal no. 44
• morphology: Neuroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian neuroblastoma

Cell Line Description

BE(2)-C is a clonal sub-line of SK-N-BE(2) (ECCAC catalogue no. 95011815) which was isolated from bone marrow of a 22-month-old male with disseminated neuroblastoma in 1972. They are reported to be multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine. The cells show a small, refractile morphology with short, neurite-like cell processes and tend to grow in aggregates.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6
TPOX: 11
vWA: 18

Culture Medium

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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