Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

M2279

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used to culture the:
  • explants of aortic valve leaflets
  • human liver cancer cell line (HepG2) cells
  • human fetal brain progenitor-derived astrocyte cells (PDA)
  • breast cancer cell line (MCF-7)
  • human glioblastoma cell line (T98G)

Preparation Note

Supplement with 0.292 g/L L-glutamine.

also commonly purchased with this product

Product No.
Description
Pricing

recommended

Product No.
Description
Pricing

related product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

M2279PROC:
M2279-1L:
M2279-BULK:
M2279-6X1L:
M2279-100ML:
M2279-24X500ML:
M2279-500ML:
M1787-100ML-PW:
M2279-20L:
M2279-1L-PW:
M2279-10L:
M2279-6X500ML:
M2279-PROC:
M2279-PH:
M2279-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Qingyun Li et al.
Neuron, 101(2), 207-223 (2019-01-05)
Microglia are increasingly recognized for their major contributions during brain development and neurodegenerative disease. It is currently unknown whether these functions are carried out by subsets of microglia during different stages of development and adulthood or within specific brain regions. Here
Winston X Yan et al.
Nature communications, 8, 15058-15058 (2017-05-13)
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along the genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ and Sequencing (BLISS)
Hiroaki Mitsuhashi et al.
Biology open, 7(4) (2018-04-06)
Aberrant expression of the full-length isoform of DUX4 (DUX4-FL) appears to underlie pathogenesis in facioscapulohumeral muscular dystrophy (FSHD). DUX4-FL is a transcription factor and ectopic expression of DUX4-FL is toxic to most cells. Previous studies showed that DUX4-FL-induced pathology requires
Luciano A Masullo et al.
Nature communications, 9(1), 3281-3281 (2018-08-18)
The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging
Anna Bodzon-Kulakowska et al.
Cytotechnology, 67(6), 1085-1091 (2014-05-08)
Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service