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C3138

Sigma-Aldrich

Cathepsin D from bovine spleen

lyophilized powder, ≥2.0 units/mg protein

Synonym(s):

CTSD

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About This Item

CAS Number:
9025-26-7
Enzyme Commission number:
3.4.23.5 (BRENDA, IUBMB)
MDL number:
MFCD00130749
UNSPSC Code:
12352204
NACRES:
NA.32

biological source

bovine spleen

Quality Level

200

Assay

10—70% protein (biuret)

form

lyophilized powder

specific activity

≥2.0 units/mg protein

mol wt

~45 kDa

manufacturer/tradename

Sigma-Aldrich

technique(s)

activity assay: suitable

color

dark brown

suitability

suitable for molecular biology

UniProt accession no.

P80209

application(s)

life science and biopharma

storage temp.

−20°C

Gene Information

cow ... CTSD(282883)

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General description

Research area: Cell Signaling

Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.

Application

Cathepsin D from bovine spleen has been used:
  • in in vitro dose-dependent fluorometric activity assays.
  • in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
  • for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
  • in cathepsin D activity assay.

Biochem/physiol Actions

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Unit Definition

One unit will produce an increase in A280 of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).

Physical form

Lyophilized powder containing citrate buffer salts

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C3138-VAR:
C3138-5UN:
C3138-BULK:
C3138-40UN:
C3138-20UN:
C3138-10UN:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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PloS one, 7(12), e49635-e49635 (2012-12-29)
Lysosomal enzymes function optimally in acidic environments, and elevation of lysosomal pH can impede their ability to degrade material delivered to lysosomes through autophagy or phagocytosis. We hypothesize that abnormal lysosomal pH is a key aspect in diseases of accumulation
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Journal of proteome research, 12(9), 3934-3943 (2013-08-02)
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