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Safety Information

C2970

Sigma-Aldrich

Monoclonal Anti-Cathepsin L antibody produced in mouse

clone 33/2, ascites fluid

Synonym(s):

Anti-CATL, Anti-CTSL, Anti-MEP

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About This Item

MDL number:
MFCD01321805
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

33/2, monoclonal

mol wt

antigen (human cathepsin L) 25 kDa
antigen (human procathepsin L) 42 kDa

contains

15 mM sodium azide

species reactivity

mouse, mink, human, rat

technique(s)

immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:200 using whole cell extract of a cultured mink lung cell line

isotype

IgG1

UniProt accession no.

P07711

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CTSL1(1514)
mouse ... Ctsl(13039)
rat ... Ctsl1(25697)

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General description

Cathepsin L is a cysteine protease and is secreted by numerous transformed cells in its inactive pre-form. It is ubiquitously expressed and is localized in nucleus. Cathepsin L have substrate interacting region located in L and R domain loops.

Specificity

Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D.

Immunogen

procathepsin L isolated from the human lung cancer cell line EPLC 32M1.

Biochem/physiol Actions

Cathepsin L expression is correlated with the metastatic potential of transformed cells. Cathepsin L is capable of degrading protein constituents of the extracellular matrix, this enzyme is thought to play a crucial role in tumor progression, metastasis and other disorders where the destruction of the matrix is the major cause of disease.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C2970-VAR:
C2970-.5ML:
C2970-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cathepsin-L influences the expression of extracellular matrix in lymphoid organs and plays a role in the regulation of thymic output and of peripheral T cell number
Lombardi G, et al.
Journal of Immunology, 174(11), 7022-7032 (2005)
Cysteine cathepsins: from structure, function and regulation to new frontiers
Turk V, et al
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1824(1), 68-88 (2012)
Increased expression and activity of nuclear cathepsin L in cancer cells suggests a novel mechanism of cell transformation
Goulet B, et al.
Molecular Cancer Research, 5(9), 899-907 (2007)
Cathepsin L1, the Major Protease Involved in Liver Fluke (Fasciola hepatica) virulence propeptide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells
Collins PR, et al.
Test, 279(17), 17038-17046 (2004)
Marouan Abouhamed et al.
American journal of physiology. Renal physiology, 290(6), F1525-F1533 (2006-02-02)
The H+-coupled polyligand transport protein divalent metal transporter 1 (DMT1) plays a key role in mammalian iron homeostasis. It has a widespread pattern of expression including tissues associated with iron acquisition and storage. Interestingly, it is also highly expressed in
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