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A6667

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD00162788
UNSPSC Code:
12352203
NACRES:
NA.54

biological source

goat

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody can be used to detect immune-complexes. The product can also be used in direct ELISA. Goat anti- rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody reacts specifically with all rabbit immunoglobulins.

Immunogen

Purified rabbit IgG.

Application

Anti-rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody can be used in immunoprecipitation and western blotting.
Proteins from lysed Leishmania amazonensis cells were analyzed by western blot using HRP-conjugated goat anti-rabbit Fc specific IgG as the secondary antibody.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A6667-VAR:
A6667PROC:
A6667-.5ML:
A6667-BULK:
A6667-1ML:
A6667-.25ML:

Certificates of Analysis (COA)

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Oleg G Chepurny et al.
American journal of physiology. Endocrinology and metabolism, 298(3), E622-E633 (2009-12-17)
Potential insulin secretagogue properties of an acetoxymethyl ester of a cAMP analog (8-pCPT-2'-O-Me-cAMP-AM) that activates the guanine nucleotide exchange factors Epac1 and Epac2 were assessed using isolated human islets of Langerhans. RT-QPCR demonstrated that the predominant variant of Epac expressed
E M Benkhart et al.
Journal of immunology (Baltimore, Md. : 1950), 165(3), 1612-1617 (2000-07-21)
IL-10 is a unique cytokine because it is anti-inflammatory and immunosuppressive. IL-10 is regulated at the level of transcription, but the critical motifs and the relevant transcription factors controlling this gene have remained elusive to date. We now report that
María P Iturriaga et al.
Oncology letters, 14(2), 2198-2206 (2017-08-07)
Cyclooxygenase (COX)-2 expression is positively correlated with malignant features in canine mammary carcinomas. Non-steroidal anti-inflammatory drugs (NSAIDs) inhibit COX activity and may therefore possess anticancer effects. Meloxicam is an NSAID that is widely used in human and veterinary medicine. High
Jae Hoon Jeong et al.
Tuberculosis and respiratory diseases, 80(3), 247-254 (2017-07-28)
Airway epithelial cells are the first line of defense, against pathogens and environmental pollutants, in the lungs. Cellular stress by cadmium (Cd), resulting in airway inflammation, is assumed to be directly involved in tissue injury, linked to the development of
Yuliya Klymenko et al.
Neoplasia (New York, N.Y.), 19(7), 549-563 (2017-06-12)
Epithelial ovarian carcinoma spreads via shedding of cells and multicellular aggregates (MCAs) from the primary tumor into peritoneal cavity, with subsequent intraperitoneal tumor cell:mesothelial cell adhesion as a key early event in metastatic seeding. Evaluation of human tumor extracts and
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