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A3672

Sigma-Aldrich

Azurin

from Pseudomonas aeruginosa, lyophilized powder

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About This Item

CAS Number:
12284-43-4
EC Number:
235-551-8
MDL number:
MFCD00130594
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

Pseudomonas aeruginosa

Quality Level

200

form

lyophilized powder

composition

Protein, ≥65% Lowry

concentration

≥65.0% (Lowry)

technique(s)

toxicology assay: suitable

solubility

water: soluble 1—1.1 mg/mL, clear, blue (light blue to blue)

UniProt accession no.

P00282

storage temp.

−20°C

Gene Information

Pseudomonas aeruginosa ... AZU(878046)

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General description

Research area: Apoptosis. Azurinis a periplasmic protein and is a homotetramer.

Application

Azurin has been used:
  • in the cytotoxicity and cell viability studies in human osteosarcoma cell line
  • for the functionalization of silicon nitride cantilevers for interaction studies
  • for coating gold surface and insulating functionalized oxide surfaces of silicon oxide and mica

Biochem/physiol Actions

Azurin acts as an electron donor for nitrite reductase in bacterial denitrification process. It exhibits anticancer activity as it hampers various independent signaling pathways associated with cancer progression. It binds to tumor suppressor protein p53 and induces cancer cell apoptosis or stalls cancer cell growth. Azurin disrupts angiogenesis by reducing the activity of VEGFR-2tyrosine kinase thereby inhibiting tumor growth. It has been observed to show cytotoxicity in human breast cancer cells and human melanoma cells.
Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition.

Physical form

Lyophilized powder containing ammonium acetate buffer salts.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A3672-VAR:
A3672-1MG:
A3672-BULK:
A3672-1MG-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
106M4040V
11140124
TEST
060M4095
118K4047

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Yunlei Zhang et al.
Applied and environmental microbiology, 78(21), 7603-7610 (2012-08-28)
Many studies have demonstrated that intravenously administered bacteria can target and proliferate in solid tumors and then quickly be released from other organs. Here, we employed the tumor-targeting property of Escherichia coli Nissle 1917 to inhibit mouse B16 melanoma and
Krzysztof Kazimierczuk et al.
Journal of magnetic resonance (San Diego, Calif. : 1997), 223, 1-10 (2012-09-11)
The resolution of multidimensional NMR spectra can be severely limited when regular sampling based on the Nyquist-Shannon theorem is used. The theorem binds the sampling rate with a bandwidth of a sampled signal and thus implicitly creates a dependence between
Bacterial redox protein azurin induce apoptosis in human osteosarcoma U2OS cells
Yang DS, et al.
Pharmacological Research, 52(5), 413-421 (2005)
Ajay Kumar Yagati et al.
Biosensors & bioelectronics, 40(1), 283-290 (2012-08-14)
We developed a nanoscale memory device consisting of signal-responsive biomaterial, which is capable of switching physical properties (such as electrical/electrochemical, optical, and magnetic) upon application of appropriate electrical signals to perform memory switching. Here, we propose a highly robust surface-confined
Daniel Raimunda et al.
Metallomics : integrated biometal science, 5(2), 144-151 (2013-01-29)
Pseudomonas aeruginosa, an opportunistic pathogen, has two transmembrane Cu(+) transport ATPases, CopA1 and CopA2. Both proteins export cytoplasmic Cu(+) into the periplasm and mutation of either gene leads to attenuation of virulence. CopA1 is required for maintaining cytoplasmic copper levels
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