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A3076

Sigma-Aldrich

Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgE, Monoclonal Anti-Human IgE−Alkaline Phosphatase antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GE-1, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:6,000
dot blot: 1:1,000

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Binds human IgE; does not bind human IgG, IgA or IgM.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for :
  • immunoblot assays
  • direct ELISA
  • dot blot
To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Biochem/physiol Actions

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Analysis Note

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A3076-.5ML:
A3076-.2ML:
A3076-BULK:
A3076-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kyoung Yong Jeong et al.
Clinical and diagnostic laboratory immunology, 11(2), 320-324 (2004-03-12)
Chironomids are widely and abundantly distributed in the vicinity of standing waters. Larvae of Chironomus and some other genera are known to contain hemoglobins, which have been described as a major allergen, and the adults that have no hemoglobins also
Nathalie Acevedo et al.
PloS one, 8(11), e78353-e78353 (2013-11-14)
Helminth infections and allergy have evolutionary and clinical links. Infection with the nematode Ascaris lumbricoides induces IgE against several molecules including invertebrate pan-allergens. These antibodies influence the pathogenesis and diagnosis of allergy; therefore, studying parasitic and non-parasitic allergens is essential
A Diaz-Perales et al.
The Journal of allergy and clinical immunology, 102(1), 127-133 (1998-07-29)
Several foods associated with the latex-fruit syndrome present relevant allergens of around 30 kd. Neither these components nor any other responsible for the reported cross-reactions have been identified and purified. We sought to isolate and characterize the 30 kd allergens
Astrid R R Heutelbeck et al.
International archives of occupational and environmental health, 82(9), 1123-1131 (2009-02-25)
Cattle are an important source of allergens in the working area of farmers. Asthma caused by cow allergens is a significant occupational problem. Yet in allergological testing, the results of in vivo and in vitro diagnostic tests are often inconsistent
Marta Burzyńska et al.
Acta scientiarum polonorum. Technologia alimentaria, 19(1), 15-24 (2020-04-01)
Small amounts of protein can be found in honey, including well known allergen sources, such as plant pollen and honeybee secretions. Despite this, there are few case reports describing allergic reactions following the consumption of honey. The aim of this
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