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94072

Sigma-Aldrich

Phalloidin–Atto 565

suitable for fluorescence, ≥80.0% (HPLC)

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

Assay

≥80.0% (HPLC)

form

solid

mol wt

Mw 1394 g/mol

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol

UV absorption

λ: 562.0-568.0 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 565 is a novel fluorescent label that belongs to the class of Rhodamine dyes. It shows a strong absorption, high fluorescence quantum yield, high thermal and photostability, and a very little triplet formation. Atto 565 consists of a mixture of two isomers with practically identical optical absorption and emission Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

94072-10NMOL:

Certificates of Analysis (COA)

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Atto 565

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