07373

Phalloidin–Atto 740

BioReagent, suitable for fluorescence

• Synonym(s): Atto 740–Phalloidin
• UNSPSC Code: 12352108
• NACRES: NA.32

Properties

• product line: BioReagent
• Assay: ≥90.0% (HPLC)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• transmittance: 254 nm; 740 nm
• fluorescence: λ_ex 750 nm; λ_em 764 nm in 0.1 M phosphate pH 7.0
• UV absorption: λ: 739 -745 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

Atto 740 belongs to a new generation of fluorescent labels for the near infrared spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the dye are strong absorption and good fluorescence as well as excellent thermal and photo-stability. Atto 740 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of ⁺¹.
Atto 740 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the reaction.
Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10^-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

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Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable