Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

58030

Sigma-Aldrich

Iodonitrotetrazolium chloride

BioReagent, ≥97.0% (calc. on dry substance, NT)

Synonym(s):

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C19H13ClIN5O2
CAS Number:
146-68-9
Molecular Weight:
505.70
Beilstein:
4093224
EC Number:
205-676-2
MDL number:
MFCD00011961
UNSPSC Code:
12352102
PubChem Substance ID:
57651636
NACRES:
NA.25

product line

BioReagent

Quality Level

200

Assay

≥97.0% (calc. on dry substance, NT)

mp

240 °C (dec.) (lit.)
~245 °C (dec.)

solubility

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

application(s)

histology

SMILES string

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChI key

JORABGDXCIBAFL-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Application

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.
Electron acceptor for the colorimetric assay of various dehydrogenases

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H228,H335

Hazard Classifications

Flam. Sol. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

58030-1G-F:4548173995212
58030-VAR-F:
58030-BULK-F:
58030-25G-F:4548173995229
58030-5G-F:4548173995236

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Christopher Auger et al.
Electrophoresis, 33(7), 1095-1101 (2012-04-28)
Phosphoenolpyruvate (PEP)-dependent kinases are central to numerous metabolic processes and mediate the production of adenosine triphosphate (ATP) by substrate-level phosphorylation (SLP). While pyruvate kinase (PK, EC: 2.7.1.40), the final enzyme of the glycolytic pathway is critical in the anaerobic synthesis
Matthildi Valianou et al.
Scientific reports, 9(1), 3015-3015 (2019-03-01)
Tuberous Sclerosis Complex (TSC) and Lymphangioleiomyomatosis (LAM) are caused by inactivating mutations in TSC1 or TSC2, leading to mTORC1 hyperactivation. The mTORC1 inhibitors rapamycin and analogs (rapalogs) are approved for treating of TSC and LAM. Due to their cytostatic and
Antoine Picciocchi et al.
The Journal of biological chemistry, 286(32), 28357-28369 (2011-06-11)
Flavocytochrome b(558) (cytb) of phagocytes is a heterodimeric integral membrane protein composed of two subunits, p22(phox) and gp91(phox). The latter subunit, also known as Nox2, has a cytosolic C-terminal "dehydrogenase domain" containing FAD/NADPH-binding sites. The N-terminal half of Nox2 contains
Yang Hongwei et al.
Ecotoxicology and environmental safety, 59(2), 249-255 (2004-08-26)
Anaerobic biodegradability of organic compounds can be assessed from the changes in organic compounds, the end products, and the activity of microorganisms. In this study, all three of these variables were considered to integrate assessment of the anaerobic biodegradability of
Sergio Ravasio et al.
Biochemistry, 41(25), 8120-8133 (2002-06-19)
The properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803 were determined by means of kinetic and spectroscopic approaches in comparison to those exhibited by the bacterial NADPH-dependent enzyme form. The ferredoxin-dependent enzyme was found to be similar to
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service