Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

56575

Sigma-Aldrich

GenElute™-LPA

Neutral carrier for ethanol precipitation

Synonym(s):

linear polyacrylamide

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

Quality Level

200

storage temp.

2-8°C

Application

GenElute™-LPA has been used to:
  • extract DNA from cyanobacterial and algal cultures
  • precipitate genomic DNA
  • extract and purify DNA from snap-frozen uveal melanoma tissues
  • to extract RNA from isolated CD4+ cells

Biochem/physiol Actions

The presence of LPA during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs, whereas most of the DNA is lost if no carrier is used. Very short DNA fragments, less than 20 base pairs, do not coprecipitate with LPA, allowing separation of labeled DNA from unreacted nucleotides by precipitation after the labeling reaction.

Packaging

GenElute LPA is tested for nucleases, and is supplied in nuclease-free water as a 25 mg/ml solution.

Principle

GenElute LPA Linear Polyacrylamide is an efficient neutral carrier for precipitating picogram amounts of nucleic acids with ethanol. The nucleic acid precipitate can be collected simply by centrifugation. LPA offers several advantages for recovering DNA or studying DNA-protein interactions, relative to other carriers, such as tRNA or glycogen. tRNA interferes with DNA during phosphorylation with polynucleotide kinase and glycogen competes with protein in DNA-protein interaction studies. In contrast, LPA is completely inert. LPA is synthesized chemically and, therefore, is not contaminated with biological material. The precipitate is visible immediately upon addition of LPA, thus eliminating wait time and low temperature incubation.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

56575-VAR-PW:
56575-1ML-PW:
56575-PH:
56575-VAR:
56575:
56575-BULK:
56575-1ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Quantitative PCR enumeration of total/toxic Planktothrix rubescens and total cyanobacteria in preserved DNA isolated from lake sediments.
Savichtcheva O
Applied and Environmental Microbiology, 77(24), 8744-8753 (2011)
Peripheral immune responses in pregnant cattle following Neospora caninum infection.
Rosbottom A
Parasite Immunology, 29(4), 219-228 (2007)
Whole-genome microarray detects deletions and loss of heterozygosity of chromosome 3 occurring exclusively in metastasizing uveal melanoma.
Lake SL
Investigative Ophthalmology & Visual Science, 51(10), 4884-4891 (2010)
Promoter de-methylation of cyclin D2 by sulforaphane in prostate cancer cells.
Hsu A
Clinical epigenetics, 3, 3-3 (2011)
Ethanol precipitation of DNA with linear polyacrylamide as carrier.
C Gaillard et al.
Nucleic acids research, 18(2), 378-378 (1990-01-25)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service