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40709

Sigma-Aldrich

Atto 590-Streptavidin

BioReagent, suitable for fluorescence

Synonym(s):

Streptavidin-Atto 590

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About This Item

MDL number:
MFCD04040679
UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Atto 590 has been coupled to streptavidin to enable all kinds of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays. Streptavidin conjugation of Atto 590 does not significantly change the labels spectral data regarding excitation and emission maxima. Given the optimized Dye/Protein-ratio molar absorbance and quantum yields remain high within application. Conjugates of our Atto dyes generally do not show strong fluorescence in unbound state, but fluorescence is boosted again as soon as they are bound to biotin. This may help overcome problems by unspecific binding which may sometimes appear with streptavidin based assays in special cases.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

40709-1MG-F:
40709-1MG-BULK-F:
40709-BULK-F:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

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Nano letters, 10(6), 2192-2196 (2010-05-21)
Semiconductor quantum dots (QDs) are used for the optical analysis of casein kinase (CK2) or the hydrolytic activity of alkaline phosphatase (ALP). Two schemes for the analysis of CK2 by a FRET-based mechanism are described. One approach involves the CK2-catalyzed
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Iron-platinum nanoparticles embedded in a poly(methacrylic acid) (PMA) polymer shell and fluorescently labeled with the dye ATTO 590 (FePt-PMA-ATTO-2%) are investigated in terms of their intracellular localization in lung cells and potential to induce a proinflammatory response dependent on concentration
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Journal of combinatorial chemistry, 11(1), 146-150 (2008-12-17)
To screen one-bead-one-compound (OBOC) combinatorial libraries, tens of thousands to millions of compound beads are first mixed with a target molecule. The beads that interact with this molecule are then identified and isolated for compound structure determination. Here we describe
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Testosterone (T), predominantly acting through its derivative 17β-estradiol (E2), regulates the brain's sexual differentiation in rodents during the perinatal sensitive period, which mirrors the window of vulnerability to the adverse effects of general anesthetics. The mechanisms of anesthesia's adverse effects
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