Merck
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650498

Sigma-Aldrich

Chloroform

HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer

Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein:
1731042
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.04

grade

HPLC Plus
for residue analysis

Quality Level

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.9%

form

liquid

contains

amylenes as stabilizer

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

impurities

≤0.02% water
≤0.5 ppb Fluorescence (quinine) at 365 nm

evapn. residue

≤0.0001%

halogenated residue

<10 ng/L (as heptachlor epoxide)

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.48 g/mL at 25 °C
1.492 g/mL at 25 °C (lit.)

λ

H2O reference

UV absorption

λ: 245 nm Amax: 1.00
λ: 250 nm Amax: 0.30
λ: 275-400 nm Amax: 0.005

application(s)

food and beverages

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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General description

Chloroform is an organic chlorinated hydrocarbon (CHC) commonly used in industries as a solvent.

Application

Chloroform may be used in the following:

  • Extraction of active constituents of anti-inflammatory drugs for their analysis by attenuated total reflectance-Fourier transform infrared spectroscopic (ATR-FTIR) methods
  • Estimation of ascorbic acid, free phenolic acids, and flavonoids in extracts of chloroform, methanol, water and, acetic acid obtained from two edible leaf samples using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector (DAD)
  • Preparation of cannabis product extracts for the analysis of delta-9-tetrahydrocannabinol (THC) residues on hands of suspected consumers by ion-mobility spectrometry (IMS)
  • Investigation of different solvents to measure their recovery ratios for the extraction of acrylamide and 5-hydroxymethylfurfural (HMF) from seafood samples to develop an HPLC-MS/MS-based method for their quantification
  • Extraction of pesticides by direct-immersion single drop micro-extraction (SDME) method from mango samples for their subsequent analysis by GC-MS
  • Sample preparation in the profiling of lipids from complex biological samples using off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry

Chloroform was used in the making of silanized coverslips for DNA combing.
It may be used as:
  • Extractant in solvent extraction process.
  • A component of the binary mobile phases for liquid chromatography–atmospheric pressure photoionization-mass spectrometry (LC–APPIMS) of triacylglycerol model compounds.
  • Solvent for recrystallization.
Suitable for HPLC, spectrophotometry, environmental testing

Packaging

1, 6×1, 4, 4×4 L in glass bottle

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Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PRTR

Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

650498-1L:4548173176871 + 650498-4L:4548173176888

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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