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RDD039

Sigma-Aldrich

Imidazole

for molecular biology, ≥99% (titration), free-flowing, Redi-Dri™

Synonym(s):

1, 3-diazole, 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline

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About This Item

Empirical Formula (Hill Notation):
C3H4N2
CAS Number:
288-32-4
Molecular Weight:
68.08
Beilstein:
103853
MDL number:
MFCD00005183
UNSPSC Code:
12352200
NACRES:
NA.21

grade

for molecular biology

Quality Level

100

vapor pressure

<1 mmHg ( 20 °C)

product line

Redi-Dri™

Assay

≥99% (titration)

form

crystalline powder

quality

free-flowing

pH

6.2-7.8 (25 °C)

pKa (25 °C)

6.95

bp

256 °C (lit.)

mp

88-91 °C (lit.)

application(s)

diagnostic assay manufacturing

foreign activity

DNase, RNase and protease, none detected

SMILES string

c1c[nH]cn1

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

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General description

Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar.

Application

Imidazole has been used
  • in the lysis, wash, and elution buffer for the purification of histidine tagged proteins
  • in gradient for the purification of histidine tagged proteins using nickel affinity chromatography
  • as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
Excellent for buffers in the range of pH 6.2-7.8

Biochem/physiol Actions

Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).

Features and Benefits

  • Qualified for molecular biology applications, DNase, Rnase, and protease free
  • Redi-Dri™ packaging controls moisture, eliminating clumps
  • Crystalline powder remains free-flowing and much easier to use
  • Safer to handle and weigh out for routine use in lab
  • Less waste, loss of drying, while maintaining high quality

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Statements

H302,H314,H360D

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

293.0 °F - closed cup

Flash Point(C)

145 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

RDD039-BULK:
RDD039-100G:
RDD039-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG7433

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