68268

Imidazole buffer Solution

BioUltra, 1 M in H₂O

• Synonym(s): Imidazole solution, Glyoxaline solution
• Empirical Formula (Hill Notation): C₃H₄N₂
• CAS Number: 288-32-4
• Molecular Weight: 68.08
• Beilstein: 103853
• MDL number: MFCD00005183
• PubChem Substance ID: 57652144
• NACRES: NA.25

Properties

• product line: BioUltra
• Quality Level: 200
• form: liquid
• concentration: 1 M in H₂O
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 9.5±0.5
• density: 1.01 g/mL at 20 °C
• SMILES string: c1c[nH]cn1
• InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
• InChI key: RAXXELZNTBOGNW-UHFFFAOYSA-N

Description

General description

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Safety Information

• Pictograms: GHS05,GHS08
• Signal Word: Danger
• Hazard Statements: H314 - H360D
• Precautionary Statements: P201 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 + P310 - P308 + P313
• Hazard Classifications: Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves