Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

H23830

Sigma-Aldrich

HEPES

≥99% (titration), crystalline, reagent grade

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
MDL number:
UNSPSC Code:
12161700
NACRES:
NA.25

product name

HEPES, reagent grade, ≥99% (titration), crystalline

biological source

synthetic

Quality Level

grade

reagent grade

Assay

≥99% (titration)

form

crystalline

storage condition

(Tightly closed. Dry)

impurities

≤1% water

color

white

useful pH range

6.8-8.2

pKa (25 °C)

7.5

mp

212.6 °C

solubility

water: 1 M, clear, colorless

density

1.44 g/cm3 at 20 °C

cation traces

Fe: <5 ppm

storage temp.

room temp

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a zwitterionic N-substituted aminosulfonic acid buffer with exceptional buffering capacity (pH 6.8-8.2, pKa 7.48 at 25°C). Recognized for versatility, it is extensively employed in cell biology, biochemical, and biological research. Its noteworthy feature lies in its neutrality towards metal ions, making it a preferred "non-coordinating buffer" suitable for solutions containing metals without affecting their activity. In cell biology studies, HEPES excels at maintaining stable physiological pH despite carbon dioxide fluctuations. It outperforms bicarbonate buffers commonly used in this context.

HEPES extends its utility as a preferred separator in creating pH gradients for isoelectric focusing, a technique in protein separation. Beyond these applications, it proves beneficial in immunoprecipitation, cell lysis, live cell imaging, establishing its indispensability across diverse research areas due to its versatility and minimal interaction with other molecules.

Application

HEPES has been used:
  • as a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
  • as a component of Hanks′ solution used in the degradation testing of Iron
  • as a component of HEPES medium in the transfection of cell culture with plasmids
  • along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
  • as a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

Features and Benefits

  • High purity product for biochemical and biological research
  • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C

  • Broad buffer range between pH 6.8 to 8.2
  • Zwitterionic at biological pH
  • High solubility and stability
  • Low UV and visible light spectrum absorbance
  • Negligible metal ion binding
  • Suitable for cell culture, electrophoresis, in vitro methods, etc.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

H23830-500G:
H23830-25KG:
H23830-5KG:
H23830-100G:
H23830-50KG:
H23830-10KG:
H23830-BULK:
H23830-1KG:
H23830-VAR:


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service