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391340

Millipore

HEPES

≥99% (alkalimetric), solid, pH 6.8-8.2, Molecular Biology

Synonym(s):

HEPES, N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
MDL number:
UNSPSC Code:
12161704
NACRES:
NA.25

product name

HEPES, Free Acid, Molecular Biology Grade, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

grade

Molecular Biology

Quality Level

Assay

≥99% (alkalimetric)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 400 mg/mL
aqueous base: soluble

cation traces

heavy metals: ≤0.0005%

foreign activity

DNase, RNase, and protease, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

Application


  • Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides: This innovative study demonstrates the application of molecular biology grade HEPES in the formulation of bioactive probes for the fluorescent labeling of root cap cells. Using cellulose biosynthesis inhibition herbicides as a model system, this research provides valuable insights into plant biology, aiding in the understanding of plant cell wall structures and functions. The use of HEPES helps maintain the physiological pH during the experiments, ensuring the stability and effectiveness of the chemical probes used (Tamogami S et al., 2021).

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

391340-25GM:
US2391340-25GM:
391340-250GM:
US2391340-250GM:
391340-25RGM:
391340-250RGM:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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