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Safety Information

G6257

Sigma-Aldrich

Glutaraldehyde solution

Grade II, 25% in H2O

Synonym(s):

Glutaric dialdehyde solution, Pentane-1,5-dial

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About This Item

Linear Formula:
OHC(CH2)3CHO
CAS Number:
Molecular Weight:
100.12
Beilstein:
605390
MDL number:
UNSPSC Code:
12352114
PubChem Substance ID:
NACRES:
NA.21

type

Grade II

Quality Level

form

liquid

concentration

25% in H2O

technique(s)

electron microscopy: suitable

color

colorless

useful pH range

2.9

mp

-10  °C ((14 °F ))

solubility

water: soluble

application(s)

cell analysis
sample preparation

SMILES string

[H]C(CCCC([H])=O)=O

InChI

1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2

InChI key

SXRSQZLOMIGNAQ-UHFFFAOYSA-N

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General description

Glutaraldehyde, known as Glutaric dialdehyde or Pentane-1,5-dial, serves as a versatile reagent with applications spanning various research domains, including cell biology and biochemical research. It finds applications as a protein crosslinker, aids in enzyme immobilization for microscopy, and contributes to histochemistry and cytochemistry processes. As an amine-reactive homobifunctional crosslinker, it acts as a cell fixative before procedures like SDS-PAGE, staining, or electron microscopy, making it a valuable tool for diverse morphological studies.

Glutaraldehyde demonstrates efficacy in crosslinking amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, it allows direct coupling of biotin hydrazides to nucleic acids, offering potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. Moreover, in histology studies, Glutaraldehyde is employed for preserving tissue sections and preparing them for detailed examination.

Application

Glutaraldehyde solution has been used:
  • for fixation of mouse and esophageal cancer cell lines
  • to crosslink amyloid-β protein
  • to crosslink gelatin in 3D scaffold preparation of trachea

Biochem/physiol Actions

Glutaraldehyde is an effective protein crosslinker and finds application in techniques like enzyme immobilisation microscopy, histochemistry and cytochemistry. It exists in different forms under acidic or neutral conditions. It is toxic to aquatic organisms. Its allergic nature leads to hypersensitivity reactions. Contact of glutaraldehyde vapors in endoscopy contributes to Colitis.

Features and Benefits

  • Versatile and adaptable for wide variety of research applications
  • Ready-made solution reduces the need for preparation time

Packaging

Not refrigerated en route

Caution

This grade is not recommended for use as an electron microscopy fixative.

Preparation Note

Not specially purified.

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Disclaimer

“The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.”

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PRTR

Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

G6257-10ML-KC:
G6257-10ML:4548173197982
G6257-1L:4548173198002
G6257-BULK:
G6257-10X10ML:4548173197999
G6257-VAR:
G6257-100ML:4548173197975


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Chemical colitis due to glutaraldehyde: case series and review of the literature
Ahishali E, et al.
Digestive Diseases and Sciences, 54(12), 2541-2541 (2009)
Neutral red uptake assay for the estimation of cell viability/cytotoxicity
Repetto G, et al.
Nature Protocols, 3(7), 1125-1125 (2008)
Initiation of apoptosis, cell cycle arrest and autophagy of esophageal cancer cells by dihydroartemisinin
Du XX, et al.
Biomedicine and Pharmacotherapy, 67(5), 417-424 (2013)
Sorption characteristics of mixed molecules of glutaraldehyde from water on mesoporous acid-amine modified low-cost activated carbon: mechanism, isotherm, and kinetics
Lloyd M, et al.
Journal of Chemistry, 2015, 790-802 (2015)
A novel tissue-engineered trachea with a mechanical behavior similar to native trachea
Park JH, et al.
Biomaterials, 62(1), 106-115 (2015)

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