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Key Documents

Safety Information

C9386

Millipore

Casein Peptone

Acid digest of casein from bovine milk, 8-12% amino, suitable for biotechnology and microbiology

Synonym(s):

Casein hydrolyzates, Hy-Case® SF, Peptone from casein, casein acid hydrolysate

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About This Item

CAS Number:
65072-00-6
EC Number:
265-363-1
MDL number:
MFCD21338741
UNSPSC Code:
41106212
NACRES:
NA.85

Product Name

Hy-Case® SF, Casein acid hydrolysate, from bovine milk

biological source

bovine milk

Quality Level

200

form

powder

nitrogen analysis

8-12% amino, ≥12.5% total

loss

≤11% loss on drying

pH

5.4-7.4

application(s)

food and beverages
microbiology

storage temp.

10-30°C

suitability

Streptococcus spp.
Streptomyces spp.

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General description

Hy-Case® SF is a high-quality acid hydrolysis of casein. Casein is a rich source of amino nitrogen so on treatment with a strong acid, it gets hydrolysed, producing free amino acids and several short peptide fragments. Special processing keeps inorganic ions to a minimum, so Hy-Case® SF is virtually a high concentration of free amino acids with a minimum of salt. It is a refined grade hydrolysate with high clarity in solution and is food grade and is recommended where a mixture of highly soluble free amino acids is required. It is especially recommended when inorganic ion levels need to be kept at a minimum.

Application

Casein hydrolysate Hy-Case® SF was used in DMCY medium, during an experimental study conducted to investigate the role of ssgA gene product in promoting the sporulation and cell division in Streptomyces griseus. It may also be used in the YH medium in studies related to Halobacterium denitrificans sp. which is characterised as being carbohydrate-utilizing, denitrifying, extremely halophilic bacteria.
Recommended for use as a microbiological nutrient in laboratory media, fermentations, and tissue culture applications requiring a mixture of highly soluble free amino acids.

Linkage

Similar to Hy-Case Amino, but contains a maximum of 2.0% sodium chloride

Reconstitution

Recommended concentration: 25 g/L

Legal Information

Hy-Case is a registered trademark of Kerry Group

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C9386-5KG:
C9386-500G:
C9386-VAR:
C9386-100G:
C9386-1KG:
C9386-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK3242
SLCJ2596
SLCF7529
SLCD7257
SLCC9638

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S Kawamoto et al.
Microbiology (Reading, England), 143 ( Pt 4), 1077-1086 (1997-04-01)
The ssgA gene of Streptomyces griseus B2682, when present in high copy number, results in both suppression of sporulation and fragmented growth of mycelia. Western analysis with polyclonal antibodies against the gene product (SsgA) revealed a close correlation between SsgA
G A Tomlinson et al.
International journal of systematic bacteriology, 36(1), 66-70 (1986-01-01)
Halobacterium denitrificans was one of several carbohydrate-utilizing, denitrifying, extremely halophilic bacteria isolated by anaerobic enrichment in the presence of nitrate. Anaerobic growth took place only when nitrate (or nitrite) was present and was accompanied by the production of dinitrogen.

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