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C4916

Sigma-Aldrich

5-Carboxyfluorescein diacetate

~95% purity (HPLC), powder

Synonym(s):

5-CFDA, 5-Carboxy-di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
79955-27-4
Molecular Weight:
460.39
Beilstein:
68265
MDL number:
MFCD00036872
UNSPSC Code:
12171500
PubChem Substance ID:
24892738
NACRES:
NA.47

product name

5-Carboxyfluorescein diacetate, ~95% (HPLC)

Assay

~95% (HPLC)

Quality Level

200

form

powder

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChI key

WPUZGNPQMIWOHE-UHFFFAOYSA-N

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Application

5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.

Biochem/physiol Actions

5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule.

comparable product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C4916-VAR:
C4916-100MG:
C4916-BULK:
C4916-100MG-PW:
C4916-25MG-PW:
C4916-25MG:

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Certificates of Analysis (COA)

Lot/Batch Number
BCBL2366V
059M4096V
13180814
13180814
029M4093V

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Dietary glycosaminoglycans interfere in bacterial adhesion and gliadin-induced pro-inflammatory response in intestinal epithelial (Caco-2) cells.
Laparra JM, et al.
International Journal of Biological Macromolecules, 47, 458-464 (2010)
Andreas W Sonesson et al.
Colloids and surfaces. B, Biointerfaces, 61(2), 208-215 (2007-09-25)
The adsorption and activity of Thermomyces lanuginosus lipase (TLL) was measured with dual polarization interferometry (DPI) and confocal microscopy at a hydrophilic and hydrophobic surface. In the adsorption isotherms, it was evident that TLL both had higher affinity for the
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