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Safety Information

B3883

Millipore

Bile bovine

dried, unfractionated

Synonym(s):

Ox gall powder

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About This Item

CAS Number:
8008-63-7
EC Number:
232-369-0
MDL number:
MFCD00130641
UNSPSC Code:
41106212
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

dried, unfractionated

color

yellow-brown

solubility

water: soluble 50 mg/mL

application(s)

microbiology

General description

Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.

Application

Bile bovine has been used:
  • in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition
  • as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology
  • as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583

Biochem/physiol Actions

Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

B3883-500G:
B3883-100G:
B3883-VAR:
B3883-25G:
B3883-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000282722
0000294640
0000278762
0000278763
0000282723

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Bile acid patterns in commercially available oxgall powders used for the evaluation of the bile tolerance ability of potential probiotics
Hu PL, et al.
PLoS ONE, 13(3), e0192964-e0192964 (2018)
Transcriptional responses of Enterococcus faecalis V583 to bovine bile and sodium dodecyl sulfate
Solheim M, et al.
Applied and Environmental Microbiology, 73(18), 5767-5774 (2007)
Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties
Guo XH, et al.
Anaerobe, 16(4), 321- 326 (2010)
Impact of bile salt adaptation of Lactobacillus delbrueckii subsp. lactis 200 on its interaction capacity with the gut
Burns P, et al.
Research in Microbiology, 162(8), 782-790 (2011)
Xin Wen et al.
Food chemistry, 257, 36-43 (2018-04-07)
As hydrolysis of carotenoid esters is believed to be highly efficient in vivo, their insufficient hydrolysis in in vitro-digestion models, particularly, regarding zeaxanthin diesters, is a current issue. Therefore, in this study, several factors related to the enzymatic hydrolysis were
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