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ATG004

A375 NY−ESO−1 Antigen Panel Cell Lines

Name: A375 NY−ESO−1 Antigen Panel Cell Lines
Brand: SIAL

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About This Item

UNSPSC Code:

41106514

NACRES:

NA.41

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biological source

human skin (malignant melanoma)

growth mode

adherent

morphology

epithelial

shipped in

dry ice

storage temp.

−196°C

General description

This product is a panel of three (3) genetically modified cell lines involving the NY-ESO-1 gene. The panel consists of a NY-ESO-1 knockout cell line (NY-ESO-1^-/-) as well as a NY-ESO-1^low and a NY-ESO-1^high expression cell line. The NY-ESO-1^-/- cell line was generated using CompoZr^® Zinc Finger Nuclease technology, and the two NY-ESO-1 expression cell lines were generated using MISSION^® lentivirus. The NY-ESO-1^low and NY-ESO-1^high cell lines express NY-ESO-1 at low and high levels relative to the NY-ESO-1^-/- cell line (NY-ESO-1^high > NY-ESO-1^low> NY-ESO-1^-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type A375 cells demonstrate NY-ESO-1^medium expression as measured via FACS.

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 88113005.

Cell Line Description

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Features and Benefits

The A375 NY-ESO-1 antigen panel cell lines feature NY-ESO-1 expressed at varying levels (KO, low, and high). These cell lines enable researchers to screen and evaluate the efficacy and potency of NY-ESO-1-targeted immunotherapies in a biologically-relevant cellular research model.

Components

ATG004A-1VL: A375 NY-ESO-1 Knockout
ATG004B-1VL: A375 NY-ESO-1 Low
ATG004C-1VL: A375 NY-ESO-1 High

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.

Legal Information

MISSION Use Licenses Agreement

ZFN Use Licenses Agreement

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.