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89747

Millipore

Tinsdale Agar Base

NutriSelect® Plus, suitable for microbiology

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
L-cystine, 0.24 g/L
peptic digest of animal tissue, 20 g/L
sodium chloride, 5 g/L
sodium thiosulfate, 0.43 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

application(s)

clinical testing
environmental
food and beverages
veterinary
microbiology

suitability

selective and differential for Corynebacterium spp.

Application

Tinsdale Agar is used for the primary isolation and identification of Corynebacterium diptheriae.
The medium differentiates between C. diphtheriae and the diphtheroids found in the upper respiratory tract. This differentiation was based on the ability of C. diphtheriae to produce grayish-black colonies, surrounded by a brown/black halo. Diphtheroids do not have this ability. The dark halo is due to the production of H2S from cystine and sodium thiosulfate, interacting with the tellurite salt.

Preparation Note

Suspend 40.7g of agar base in 1000ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to 50°C and add aseptically the contents of 1 unit (part A+B) of Tinsdale Supplement (Cat. No. 73831). Mix throughly and pour into sterile dishes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

89747-VAR-F:
89747-BULK-F:
89747-500G-F:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD9839
BCBW5015
BCBT5172
BCBP8788V
BCBG6297V

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