80457

Supelco

Trifluoroacetic acid

eluent additive for LC-MS, LiChropur, ≥99.0% (GC)

Synonym(s):
TFA
Linear Formula:
CF3COOH
CAS Number:
Molecular Weight:
114.02
Beilstein/REAXYS Number:
742035
EC Number:
MDL number:

Quality Level

grade

eluent additive for LC-MS

vapor density

3.9 (vs air)

vapor pressure

97.5 mmHg ( 20 °C)

assay

≥99.0% (GC)

application(s)

HPLC: suitable
LC/MS: suitable

impurities

≤0.05% water
≤50 mg/kg total sulfur (as SO4)

refractive index

n20/D 1.3 (lit.)

bp

72.4 °C (lit.)

mp

−15.4 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfite (SO32-): ≤10 mg/kg

cation traces

Ag: ≤0.05 mg/kg
Al: ≤0.1 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.5 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.1 mg/kg
Co: ≤0.05 mg/kg
Cr: ≤0.1 mg/kg
Cu: ≤0.05 mg/kg
Fe: ≤1.0 mg/kg
K: ≤1.0 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤1.0 mg/kg
Mn: ≤0.1 mg/kg
Mo: ≤0.05 mg/kg
NH4+: ≤10 mg/kg
Na: ≤1.0 mg/kg
Ni: ≤0.1 mg/kg
Pb: ≤0.05 mg/kg
Sr: ≤0.05 mg/kg
Tl: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

UV absorption

λ: 260 nm Amax: ≤0.9
λ: 270 nm Amax: ≤0.1
λ: 280 nm Amax: ≤0.05
λ: 290 nm Amax: ≤0.04
λ: 300 nm Amax: ≤0.03
λ: 320 nm Amax: ≤0.025

SMILES string

OC(C(F)(F)F)=O

suitability

passes test for LC-MS

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

DTQVDTLACAAQTR-UHFFFAOYSA-N

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General description

Trifluoroacetic acid is commonly used as a mobile phase additive in liquid chromatography-mass spectrometry (LC-MS).
Features:
  • Extensive QC testing ensures the highest specifications.        
  • Optimized to improve ionization and resolution.        
  • Extremely low levels of inorganic and organic impurities.       
  • Manufactured specifically for accurate and fast LC-MS.

Application

Trifluoroacetic acid may be used as a mobile phase additive for the analysis of basic compounds in biological samples using hydrophilic interaction liquid chromatography-electrospray ionization coupled to tandem mass spectrometry (HILIC–ESI/MS/MS) and reversed-phase liquid chromatography coupled to tandem mass spectrometry (RP-LC-MS/MS).

Packaging

Clear borosilicate glass, high hydrolytic resistance (Type I)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

pictograms

CorrosionExclamation mark

signalword

Danger

RIDADR

UN 2699 8 / PGI

WGK Germany

WGK 2

Flash Point F

212.0 °F - Pensky-Martens closed cup

Flash Point C

> 100 °C - Pensky-Martens closed cup

Certificate of Analysis

Certificate of Origin

Simple means to alleviate sensitivity loss by trifluoroacetic acid (TFA) mobile phases in the hydrophilic interaction chromatography?electrospray tandem mass spectrometric (HILIC?ESI/MS/MS) bioanalysis of basic compounds
Shou ZW and Naidong W
Journal of Chromatography. B, Biomedical Sciences and Applications, 825(2), 186-192 (2005)
Protocols
Straightforward HPTLC-MS analysis of lactose in dairy products (milk or yoghurt) using only protein crash, centrifugation and dilution as sample preparation.
Read More

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