322415

Sigma-Aldrich

Methanol

anhydrous, 99.8%

Synonym(s):
Methyl alcohol
Linear Formula:
CH3OH
CAS Number:
Molecular Weight:
32.04
Beilstein/REAXYS Number:
1098229
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

grade

anhydrous

vapor density

1.11 (vs air)

vapor pressure

410 mmHg ( 50 °C)
97.68 mmHg ( 20 °C)

assay

99.8%

form

liquid

autoignition temp.

725 °F

expl. lim.

36 %

application(s)

tissue processing: suitable

impurities

<0.002% water
<0.005% water (100 mL pkg)

evapn. residue

<0.0003%

refractive index

n20/D 1.329 (lit.)

bp

64.7 °C (lit.)

mp

−98 °C (lit.)

density

0.791 g/mL at 25 °C (lit.)

SMILES string

CO

InChI

1S/CH4O/c1-2/h2H,1H3

InChI key

OKKJLVBELUTLKV-UHFFFAOYSA-N

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General description

Methanol is an industrial organic solvent commonly used in organic synthesis. It is being considered as a potential hydrogen source in fuel cell technology due to its high H/C ratio, low propensity for soot generation, relatively low reforming temperature and its liquid state at room temperature. The olefins (ethylene or propylene) formed from methanol via MTO (methanol-to-olefins) process, can be an alternative to oil and gas to produce hydrocarbon fuels.

Application

Methanol has been used for fixing the tissues during preparation of thin giemsa stained blood smears. It has been used in the purification step during the synthesis of pyocyanin, a toxic compound found in Pseudomonas aeruginosa.
Methanol has been used in following applications:
  • Used for the synthesis and purification of pyocyanin, spectrophotometric estimation of pyocyanin concentration, and the calculation of pyocyanin molar concentration using spectrophotometer absorption data.
  • Used as a component of staining solution used in karyotyping.
  • Used as a component of staining solution to stain cell colonies in colony formation assay.

Packaging

1, 6×1, 2, 4×2 L in Sure/Seal™
20 L in Pure-Pac™ 2
100, 12×100 mL in Sure/Seal™
200 L in Pure-Pac™ 1
18 L in Pure-Pac™ 1

Other Notes

Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.

Legal Information

Pure-Pac is a registered trademark of Sigma-Aldrich Co. LLC

signalword

Danger

hazcat

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

storage_class_code

3 - Flammable liquids

WGK Germany

WGK 2

Flash Point F

49.5 °F - closed cup

Flash Point C

9.7 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Matthew P Horning et al.
Lab on a chip, 14(12), 2040-2046 (2014-05-02)
A paper microfluidic cartridge for the automated staining of malaria parasites (Plasmodium) with acridine orange prior to microscopy is presented. The cartridge enables simultaneous, sub-minute generation of both thin and thick smears of acridine orange stained parasites. Parasites are stained...
Generation of Embryonic Stem Cells in Rabbits.
Methods in Molecular Biology, 1341, 49-66 (2016)
Rajkumar Cheluvappa
MethodsX, 1, 67-73 (2014-01-01)
Preparation of the toxin pyocyanin from the bacterium Pseudomonas aeruginosa is an exacting procedure. Pyocyanin is expensive to commercially purchase. The sellers do not give out the extraction procedure. Classically, pyocyanin preparation involves complicated multi-step P. aeruginosa culturing and solvent...
Standardized chemical synthesis of Pseudomonas aeruginosa pyocyanin.
Cheluvappa R.
Gastroenterologia y hepatologia, 22, 1350-1351 (2007)
Beyond oil and gas: the methanol economy.
Olah GA.
Angewandte Chemie (International Edition in English), 44(18), 2636-2639 (2005)

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