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Key Documents

Safety Information

SCT024

Sigma-Aldrich

BioTracker 543 Yellow β-Gal Dye

Live cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).

Synonym(s):

Live cell imaging probe

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.47

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

Related Categories

General description

β-galactosidase, also called beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. β-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. β-gal has also been used to measure cellular senescence (SA-β-gal).

The BioTracker 543 Yellow β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. Because the dye is almost non-fluorescent in the absence of β-galactosidase, this probe exhibits good S/N ratio.

Spectral Properties
Absorbance: 525nm (488nm compatible)
Emission: 543nm

Storage and Stability

Store BioTracker 543 Yellow β-Gal Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCT024:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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