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Key Documents

Safety Information

SCT021

Sigma-Aldrich

BioTracker 609 Red Ca2+ AM Dye

Live cell imaging calcium (Ca2+) indicator for neuroscience and cancer applications.

Synonym(s):

Live cell calcium detection probe, Live cell imaging probe, Red fluorescent calcium indicator

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.47

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

General description

Calcium (Ca2+) play a vital role in the physiology and biochemistry of the cell. Calcium ions play an important role in signal transduction pathways, neurotransmitter release from neurons, contraction of all muscle cell types, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable cell membranes, as well as proper bone formation.

The BioTracker 609 Red Ca2+ AM Dye is a live cell red fluorescent calcium indicator. Fluorescence intensity of this fluorescent probe reversibly changes depending on Ca2+ concentration. It has red fluorescence with maximum emission at 609 nm (Excitation 590nm) and is suitable for detection of micromolar- to submicromolar-range calcium ions. The dye is hydrolyzed by intracellular esterases to become soluble which equally distributes in the cytoplasm. This feature enables detection of intracellular calcium concentration changes with superior accuracy compared to other calcium indicators.

Spectral Properties
Absorbance: 595nm
Emission: 609nm

Storage and Stability

Store BioTracker 609 Red Ca2+ AM Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

1202.4 °F

Flash Point(C)

650.2 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCT021:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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