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SCC050

Sigma-Aldrich

PluriStem C57BL/6N Murine ES Cells

This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.

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About This Item

UNSPSC Code:
41106509
eCl@ss:
32011203
NACRES:
NA.81

biological source

mouse

Quality Level

100

manufacturer/tradename

PluriSTEM®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

General description

Embryonic Stem Cell line derived from C57BL/6N blastocysts. C57BL/6N is the most commonly used inbred mouse strain. Large scale mouse knockout projects such as NIH’s KOMP project use C57BL/6 as the standard background for mouse genome engineering. C57BL/6 is also the reference strain for mouse genome sequencing at the NHGRI. C57BL/6N mice show good breeding performance. As a commonly used reference strain, transgenic mouse models are often backcrossed to a uniform C57BL/6 background, and B6 ES cells facilitate building complex models which combine gene inactivation with existing transgenics maintained in a B6 background. Additional phenotypic information is available at the Mouse Genome Informatics website (http://www.informatics.jax.org/external/festing/mouse/docs/C57BL.shtml). The B6 ES line was genome verified by determining the alleles at 29 SNPs that are informative across a large number of inbred mouse strains.

STRAIN: C57BL/6N

GENDER: Male

KARYOTYPE: 40XY

Cell Line Description

Embryonic Stem Cells

Application

Research Category
Stem Cell Research
This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.

Packaging

2.5 x 106 cells per vial, passage 9

Physical form

Cells are supplied frozen in basal medium supplemented with 50% FCS and 10% DMSO

Storage and Stability

Place vials in liquid nitrogen immediately upon receipt until it is convenient to proceed to subculture.

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

SCC050:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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