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Key Documents

Safety Information

SF002

Sigma-Aldrich

ESGRO Complete Basal Medium, 100mL

The ESGRO Complete Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF & BMP.

Synonym(s):

Mouse ES Cell Media

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.81

Quality Level

300

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

General description

ESGRO Complete™ Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF and BMP, and during routine washing steps when passaging cells.

Application

Routine washing of cells.

Suitable for serum free culture of ES cells when used with LIF and BMP.

Suitable for use as a base medium for differentiation upon addition of differentiation factors of choice.

Medium suitable for use on feeder independent cell lines.

Physical form

Basal serum free medium formulation without BMP or LIF.

pH 7.1-7.3

Storage and Stability

Store at -20°C. Refer to lot expiration date on label. After thawing, store at 4°C for one month. DO NOT REFREEZE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

SF002-100:
SF002-500:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Qi Long Ying et al.
Cell, 115(3), 281-292 (2003-11-26)
The cytokine leukemia inhibitory factor (LIF) drives self-renewal of mouse embryonic stem (ES) cells by activating the transcription factor STAT3. In serum-free cultures, however, LIF is insufficient to block neural differentiation and maintain pluripotency. Here, we report that bone morphogenetic
A critical appraisal of factors affecting the accuracy of results obtained when using flow cytometry in stem cell investigations: where do you put your gates?
Hughes O. R. et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology null

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