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S8001U

Sigma-Aldrich

CpGenome Human Non-Methylated DNA Standard Set

It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.

Synonym(s):

Human Non-Methylated DNA Standard

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.31

Quality Level

100

species reactivity

human

manufacturer/tradename

CpGenome
Upstate®

application(s)

genomic analysis

shipped in

dry ice

General description

The CpGenome Human Non-Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ amplification kits.

Materials Provided:

One vial containing 5 µg (20 µL) of CpGenome Human Non-Methylated DNA Standard at a concentration of 250 ng/µL.

Validation:

Methylation-specific PCR (MSP) was performed on the CpGenome Human Non-Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the U primers generated product for this Standard.

CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

S8001U:

Certificates of Analysis (COA)

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