MABN390

Anti-Sigma IR Antibody, clone ICR-SIG1R-A

clone ICR-SIG1R-A, from rat

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ICR-SIG1R-A, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse (immunogen homology)
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2c
• NCBI accession no.: NP_005857
• UniProt accession no.: Q99720
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SIGMAR1(10280)

Description

General description

Sigma1R, or Sigma non-opioid intracellular receptor 1, is part of the ERG2 family that forms a ternary complex with ANK2 and ITPR3. Sigma1R is involved in lipid transport from the endoplasmic reticulum, BDNF and EGF signaling, potassium ion channel regulation and other cell functions including apoptosis, survival and proliferation. It was initially discovered through its binding to various psychoactive drugs, and has been studied in memory, learning and mood alteration processes. Sigma1R is often found in the nuclear membrane, synapses and endoplasmic reticulum of cells within liver, brain, kidney and thymus tissues.

Immunogen

Linear peptide corresponding to human Sigma IR.

Application

Detect the Sigma IR protein using this Anti-Sigma IR, clone ICR-SIG1R-A validated for use in wb, IP, ICC, Flow & IHC.

Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in certain cancer cell lysates.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.
Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in tumor cells.
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in colo-587 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sigma IR in 10 µg of colo-587 cell lysate.

Target description

~25 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing at ~25 kDa, ~23 kDa, ~21.5 kDa, ~12 kDa and ~21 kDa

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Colo-587 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable