MABF2247

Anti-ISG15 Antibody, clone 3C2

• Synonym(s): IP17, Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, Ubiquitin cross-reactive protein, Ubiquitin-like protein ISG15
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: hamster
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3C2, monoclonal
• mol wt: calculated mol wt 17.9 kDa; observed mol wt ~16 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100
• technique(s): western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: NP_056598
• UniProt accession no.: Q64339
• storage temp.: 2-8°C
• Gene Information: mouse ... Isg15(100038882)

Description

Specificity

Clone 3C2 is an Armenian Hamster monoclonal antibody that detects Ubiquitin-like protein ISG15.

Immunogen

His-tagged full-length recombinant mouse Ubiquitin-like protein ISG15.

Application

Quality Control Testing

Isotype testing: Identity confirmation by isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be Armenian hamster IgG.

Tested Applications

Western Blotting Analysis: A 1:250 dilution from a representative lot detected ISG15 in primary mouse embryonic fibroblasts infected with Chikungunya virus.

Western Blotting Analysis: A representative lot detected ISG15 in Western Blotting applications (Lenschow, D.J., et al. (2005). J Virol. 79(22):13974-83; Lenschow, D.J., et al. (2007). Proc Natl Acad Sci USA. 104(4):1371-6; Fan, J-B., et al. (2015). Sci Rep. 5:12704).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Ubiquitin-like protein ISG15 (UniProt: Q64339; also known as Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, IP17, Ubiquitin cross-reactive protein) is encoded by the Isg15 (also known as G1p2, Ucrp) gene (Gene ID: 100038882) in murine species. ISG15 is a disulfide-linked homodimeric, interferon-induced protein that plays a key role in host antiviral response. It is reported to exist in three distinct states: free form, released into the extracellular space, and as conjugated to target proteins. It contains two ubiquitin-like domains (aa 2-76 and 77-155) that are essential for its efficient conjugation to cellular proteins. Ubiquitin-like 2 domain is essential for the first two steps allowing the linking of ISG15 to the E1 and E2 enzymes while Ubiquitin-like 1 domain is essential for the E3-mediated transfer of ISG15 from the E2 to the lysine residues of the target protein. It also requires its C-terminal LRLRGG motif to form intracellular conjugates. ISG15 plays key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGlylation is reported to involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes that catalyze the conjugation of ISG15 to a lysine residue in the target protein. Some of the target proteins of ISG15 include JAK1, ERK1, ERK2, STAT5A, TRIM25, and globin. The secreted form is also known to induce natural killer cell proliferation, act as a chemotactic factor for neutrophils, and act as a IFN -inducing cytokine. The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR that leads to secretion of IFN and IL-10. S-nitrosylation of ISG15 can decrease its dimerization, thereby increasing the availability as well as the solubility of monomeric ISG15 for its conjugation to cellular proteins. (Ref.: Perng, Y-C., and Lenschow, DJ. (2018). Nat. Rev. Microbiol. 16(7); 423-439; Lenschow, DJ., et al. (2007). Proc. Natl. Acad. Sci, USA. 104(4); 1371-1376; Lenschow, DJ., et al. (2005). J. Virol. 79(22); 13974-13983).

Physical form

Purified Armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable