MABE146

Anti-5-methylcytosine Antibody, clone 33D3

clone 33D3, from mouse

• Synonym(s): methylcytosine, methylcytidine
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 33D3, monoclonal
• species reactivity: human, mouse
• technique(s): Southern blotting: suitable; dot blot: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); methylated DNA immunoprecipitation (MeDIP): suitable
• isotype: IgG1λ
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.

Specificity

Predicted to react with multiple species.

This antibody detects 5-Methylcytosine, but not unmethylated cytosine.

Immunogen

Ovalbumin-conjugated 5-methylcytosine

Application

IF
Habib, M., et al. (1999). Exp Cell Res. 249:46-53

IHC-P
Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693

Flow Cytometry
Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262

MeDIP
Keshet, I., et al. (2006). Nat Genet. 38(2):149-153

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Use Anti-5-methylcytosine Antibody, clone 33D3 (mouse monoclonal antibody) validated in DB, IF, SB, IHC(P), Flow Cytometry, MeDIP to detect 5-methylcytosine also known as methylcytosine, methylcytidine.

Quality

Evaluated by Dot Blot in methylated and unmethylated BRCA1.

Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.

Target description

Detects methylated cytosine

Linkage

Replaces: MABE527

Physical form

Format: Purified

Protein A

Purified mouse monoclonal IgG1λ in buffer containing PBS and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Methylated and unmethylated BRCA1

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable