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Safety Information

MABD150

Sigma-Aldrich

Anti-LIF Receptor, clone 1C7 (Azide Free) Antibody

clone 1C7, from mouse

Synonym(s):

Leukemia inhibitory factor receptor, LIF receptor, LIF-R, D-factor, CD118, GP190, LIFR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1C7, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
inhibition assay: suitable

isotype

IgG1κ

NCBI accession no.

NP_001106857

UniProt accession no.

P42703

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LIFR(3977)

General description

The LIF receptor is critical in modern mouse stem cell maintenance as the receptor for LIF, the embryonic stem cell (ESC) growth factor that maintains pluripotency and self renewal in mouse ES cells, however physiologically, the importance of LIF-R in real mouse development has been less clear. LIF receptor does play a signaling role and when cleaved its extracellular portion can act as a decoy receptor and block exogenous LIF from performing its function on ES cells. LIF receptor is expressed in multiple tissues in the mouse during development from the liver to the lung and brain. LIF receptor also is important in immune responses and in the stimulation of the interferon response to viral infection. Also LIF receptor has recently been identified as a metastasis suppressor that functions through the Hippo-YAP pathway in humans.

Immunogen

Recombinant protein corresponding to mouse LIF Receptor.

Application

Anti-LIF Receptor, clone 1C7 (Azide Free) is an antibody against LIF Receptor for use in FC, Inhibition.
Inhibition Assay Analysis: A representative lot detected LIF Receptor in LIF binding to JAR cells (Blanchard, F., et al. (1997) International Immunology. 9(12):1775-1784).
Inhibition Assay Analysis: A representative lot detected LIF Receptor in IL3 proliferative response in Ba/F3 cells (Pitard, V., et al. (1997) Journal of Immunological Methods. 205:177-190).
Research Category
Stem Cell Research
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Flow Cytometry in CHO D1IgD2-mycDAF cells .

Flow Cytometry Analysis: 1.0 µg of this antibody detected LIF Receptor in CHO D1IgD2-mycDAF.

Target description

123 kDa calculated

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABD150:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Separate functions for the two modules of the membrane-proximal cytokine binding domain of glycoprotein 190, the leukemia inhibitory factor low affinity receptor, in ligand binding and receptor activation.
Voisin, MB; Bitard, J; Daburon, S; Moreau, JF; Taupin, JL
The Journal of Biological Chemistry null
Epitope-function relationships of human leukemia inhibitory factor receptors using a novel set of anti-gp190 mAB.
Blanchard, F; Pitard, V; Taupin, JL; Raher, S; Hallet, MM; Moreau, JF; Godard, A; Jacques, Y
International Immunology null
V Pitard et al.
Journal of immunological methods, 205(2), 177-190 (1997-07-14)
Leukemia inhibitory factor (LIF), oncostatin-M (OSM), ciliary neurotrophic factor (CNTF) and cardiotrophin-1 (CT1) act through transmembrane receptors which share the gp190 glycoprotein chain. The understanding of its involvement in the biology of these cytokines is of importance since these systems
J L Taupin et al.
The Journal of biological chemistry, 276(51), 47975-47981 (2001-10-19)
The receptor for the cytokine leukemia inhibitory factor (LIF) associates the low affinity binding component gp190 and the high affinity converter gp130, both of which are members of the family of hematopoietic receptors characterized by the cytokine receptor homology (CRH)

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