Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

MABC004F

Sigma-Aldrich

Mouse IgG2a Negative Control, clone GC270, FITC conjugate

Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

ascites fluid

clone

GC270, monoclonal

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgG2a

shipped in

wet ice

target post-translational modification

unmodified

Specificity

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Immunogen

Protein I preparation from Neisseria gonorrhoeae.

Application

Fluorescein conjugated mouse IgG2a is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.
Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies

Physical form

Purified from mouse ascitic fluid. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Storage and Stability

Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABC004F:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

S Chinnapaiyan et al.
PloS one, 12(1), e0169161-e0169161 (2017-01-07)
Recurrent lung infections and pneumonia are emerging as significant comorbidities in the HIV-infected population in the era of combination antiretroviral therapy (cART). HIV infection has been reported to suppress nasal mucociliary clearance (MCC). Since the primary components driving nasal MCC
Eleonora Cimini et al.
Microorganisms, 7(9) (2019-09-25)
An expansion of effector/activated Vδ2 T-cells was recently described in acute Zika virus (ZIKV)-infected patients, but their role in the protective immune response was not clarified. The aim of this study was to define the antiviral activity of Vδ2 T-cells

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service