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M5409

Sigma-Aldrich

Mouse IgG2a Isotype Control from murine myeloma

clone UPC-10, purified immunoglobulin, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

UPC-10, monoclonal

form

buffered aqueous solution

technique(s)

flow cytometry: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Related Categories

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.

Specificity

When evaluated in flow cytometry, the product did not stain human peripheral blood lymphocytes (PBLs). Anti-Mouse IgG (whole molecule), F(ab′)2 fragment FITC, Catalog Number F2883, along with 1 mg of the product was incubated with human PBLs and then evaluated by flow cytometry.

Application

Mouse IgG2a Isotype Control from murine myeloma has been used in:
  • immunoprecipitation
  • enzyme-linked immunosorbent assay (ELISA)
  • fluorescence microscopy
  • indirect immunocytochemistry

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

M5409-VAR:
M5409-.1MG:
M5409-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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David M Markusic et al.
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The Immunoglobulins: Structure and Function, 575-586 (1998)
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Secchiero P, et al.
Journal of Cellular Physiology, 227(5), 1829-1837 (2012)
Molecular Genetics of Immunoglobulin, 17, 575-586 (1987)
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