MAB4334
Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2
clone UIC2, Chemicon®, from mouse
Synonym(s):
P-glycoprotein, CD243, p170, Pgp
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
UIC2, monoclonal
species reactivity
primate, human
should not react with
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... ABCB1(5243)
General description
UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE′s UIC2 Shift assay - see product ECM905).
Specificity
P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)
Immunogen
Epitope: conformational extracellular epitope
Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.
Application
Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2 detects level of MDR1 & has been published & validated for use in FC, IF, IP, IH, IH(P).
Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems.
Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.
Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.
Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.
IHC on frozen and paraffin sections: 1 - 10 μg/mL.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.
Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.
Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.
IHC on frozen and paraffin sections: 1 - 10 μg/mL.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Toxicology & Drug Resistance
Toxicology & Drug Resistance
Physical form
Format: Purified
The immunoglobulin was purified by protein A Sepharose™ chromatography and is presented as a liquid in in 0.1M PBS, containing 0.1% sodium azide as a preservative.
Storage and Stability
Maintain at 2-8°C for up 12 months from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
MAB4334:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Levels of multidrug resistance (MDR1) P-glycoprotein expression by human breast cancer correlate with in vitro resistance to taxol and doxorubicin.
Mechetner, E, et al.
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