MAB4211
Anti-CD34 Class I Antibody, clone B1-3C5
clone B1-3C5, Chemicon®, from mouse
About This Item
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
B1-3C5, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CD34(947)
Related Categories
Specificity
Cell reactivity (Tindle et al., 1985):
Normal: No reaction with normal peripheral lymphocytes, granulocytes, monocytes, erythrocytes and platelets. Reacts with <4% of cells in normal bone marrow.
Clinical: Reaction
Acute myeloid leukemia (AML, M1 and M2) positive
Null lymphoblastic leukemia (Null ALL) positive
Common acute lymphoblastic leukemia (CALL) positive
Myeloblastic and lymphoblastic crises of chronic
granulocytic leukemia (CGL) positive
Chronic myeloid leukemia (CML) negative
T cell acute lymphoblastic leukemia (TALL) negative
B cell acute lymphoblastic leukemia (BALL) negative
Chronic lymphocytic leukemia (CLL) negative
Cell lines:
Reacts with early myeloblast line KG1. No reaction with null, B or T lymphoid, erythroid or later myeloid cell lines.
Immunogen
Application
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl of buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.
Cell Structure
ECM Proteins
Physical form
Storage and Stability
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Legal Information
Disclaimer
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Regulatory Listings
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JAN Code
MAB4211:
Certificates of Analysis (COA)
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