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H3524

Sigma-Aldrich

Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse

clone LK2, ascites fluid

Synonym(s):

Anti-CPN60, Anti-GROEL, Anti-HLD4, Anti-HSP-60, Anti-HSP60, Anti-HSP65, Anti-HuCHA60, Anti-SPG13

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LK2, monoclonal

mol wt

antigen 60 kDa

contains

15 mM sodium azide

species reactivity

spinach, chicken, E. coli, helminth, rat, human

technique(s)

electron microscopy: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
western blot: 1:100 using cultured human foreskin fibroblast extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSPD1(3329)
rat ... Hspd1(63868)

General description

Monoclonal Anti-Heat Shock Protein 60 (HSP60), clone LK2, recognizes an epitope located between amino acid residues 383-419 of the human (corresponding to a.a. residues 356-393 of the mycobacterial) HSP60. The antibody is reactive against the mammalian (e.g., human, rat), avian (e.g., chicken), bacterial (e.g., E. coli), helminths and spinach HSP60. In immunoblotting, the antibody may label additional bands at approx. 38 and 97 kDa. It is reactive with both the constitutive and the inducible HSP60. It shows a raised level of staining in immunohistochemistry of formalin-fixed, paraffin-embedded synovial membranes taken from patients with juvenile chronic arthritis.
Monoclonal anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat shock protein 60.

Immunogen

recombinant human heat shock protein 60 (HSP60).

Application

Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse has been used in:western blotting, immunocytochemistry, enzyme linked immuno sorbent assay (ELISA) ,immunoblotting

Biochem/physiol Actions

A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. The 60 kDa HSP family (HSP60) which retained a uniquely high level of sequence conservation during evolution is a focus of interest as a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia. In human arthritis and experimentally-induced arthritis in animals, disease development was seen to coincide with development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homologue. A human mitochondrial protein, originally designated P1, has been described as the human homologue of the bacterial HSP60, and >45% of the protein has sequence identity with its bacterial homologue (groEL, HSP65).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

H3524-VAR:
H3524-.2ML:
H3524-.5ML:
H3524-BULK:


Certificates of Analysis (COA)

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Inge Kühl et al.
Mitochondrion, 12(3), 381-390 (2012-02-22)
Complexes III and IV of the mitochondrial respiratory chain contain a few key subunits encoded by the mitochondrial genome. In Saccharomyces cerevisiae, fifteen mRNA-specific translational activators control mitochondrial translation, of which five are conserved in Schizosaccharomyces pombe. These include homologs
OPA1 haploinsufficiency induces a BNIP3-dependent decrease in mitophagy in neurons: relevance to Dominant Optic Atrophy
Moulis MF, et al.
Journal of Neurochemistry, 140(3), 485-494 (2017)
Ann M Fallon et al.
In vitro cellular & developmental biology. Animal, 44(8-9), 351-356 (2008-07-16)
The Aedes albopictus Aa23 cell line, which is persistently infected with Wolbachia pipientis strain wAlbB, tends to grow as aggregated clusters of cells that are difficult to disperse for conventional quantification based on cell number. We used A. albopictus C7-10
Malgorzata Milewska et al.
Journal of neurochemistry, 111(4), 1022-1030 (2009-09-22)
Hereditary spastic paraplegia describes a group of neurodegenerative diseases characterized by lower limb progressive weakness and spasticity. Troyer syndrome is an autosomal recessive form of hereditary spastic paraplegia caused by a frameshift mutation (1110delA) in the SPG20 gene encoding spartin
A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression
Kuhl I, et al.
Nucleic Acids Research, 39(18), 8029-8041 (2011)

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