MAB2019Z

Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free

clone 15F11, Chemicon^®, from mouse

• Synonym(s): MAB2019
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 15F11, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• NCBI accession no.: NM_002210.2
• UniProt accession no.: P06756
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGAV(3685)

Description

Specificity

Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. The antibody binds to the alphaVbeta5 complex. The monoclonal antibody MAB2019Z has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding of the antibody to alphaVbeta5. Monoclonal antibody MAB2019Z does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.

Immunogen

Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.

Application

Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free is an antibody against Integrin αVβ5 for use in ELISA, FC, IP & IC.

Immunoprecipitation

Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.

Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Physical form

Format: Purified

Purified immunoglobulin. Liquid at 1 mg/mL in PBS, containing no preservatives.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable