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CBL162

Sigma-Aldrich

Anti-CD9 Antibody, clone MM2/57

clone MM2/57, Chemicon®, from mouse

Synonym(s):

MRP-1, DRAP-27

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

MM2/57, monoclonal

species reactivity

human, rabbit, mouse

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NM_001769.2

UniProt accession no.

P21926

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD9(928)

Related Categories

Specificity

This antibody reacts with a 25 kDa molecular weight band on gels of non-reduced platelet membranes and immunoprecipitates a similar band from Iodine125-labeled platelets. The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals localized in the cytoplasm.

Immunogen

Human platelet glycoprotein

Application

Anti-CD9 Antibody, clone MM2/57 detects level of CD9 & has been published & validated for use in FC, IP, WB, IH.
Immunohistochemistry: Frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues
Platelet inhibition and aggregation studies
Optimal working dilutions must be determined by the end user. the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Physical form

Format: Purified
Protein A purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Storage and Stability

1 year at 4°C from date of shipment

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

CBL162:
CBL162-K:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dmitry Ter-Ovanesyan et al.
eLife, 10 (2021-11-17)
Extracellular vesicles (EVs) are released by all cells into biofluids and hold great promise as reservoirs of disease biomarkers. One of the main challenges in studying EVs is a lack of methods to quantify EVs that are sensitive enough and
Anna Lischnig et al.
Molecular & cellular proteomics : MCP, 21(9), 100273-100273 (2022-08-03)
There is a long-held consensus that several proteins are unique to small extracellular vesicles (EVs), such as exosomes. However, recent studies have shown that several of these markers can also be present in other subpopulations of EVs to a similar
Olivia Ruhen et al.
Biomedicines, 9(1) (2020-12-31)
There is increasing recognition of circulating tumour DNA (ctDNA) as a non-invasive alternative to tumour tissue for the molecular characterisation and monitoring of disease. Recent evidence suggests that cancer-associated changes can also be detected in the DNA contained within extracellular
Stimulus-response coupling in human platelets activated by monoclonal antibodies to the CD9 antigen, a 24 kDa surface-membrane glycoprotein.
Carroll, R C, et al.
The Biochemical Journal, 266, 527-535 (1990)
Julia Dancourt et al.
Scientific reports, 13(1), 1086-1086 (2023-01-20)
Extracellular vesicles (EVs) are biological vehicles that are thought to mediate cell-cell communication via the transfer of biomolecules from donor to acceptor cells. Repurposing those natural vesicles into therapeutics delivery vectors is a high priority challenge for translational science. Here
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