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Safety Information

MAB1911

Sigma-Aldrich

Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8

clone 9F8, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

9F8, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TNC(3371)

Specificity

Binds to a recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 1-3.

Immunogen

Epitope: type III repeats 1-3
Purified human tenascin

Application

Immunohistochemistry at 2 μg/mL on tissues fixed with cold acetone

Immunocytochemistry at 2 μg/mL on U251 cells

Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions

ELISA: 2 x 10-10 M endpoint dilution

Affinity constant Kd= 6.37 x 10-9

Final working dilutions must be determined by end user.
This Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8 is validated for use in ELISA, WB, IC, IH for the detection of Tenascin.

Physical form

Format: Purified
Liquid in 0.02 M phosphate, 0.25 M Sodium Chloride pH 7.6, with 0.1% Sodium Azide.

Storage and Stability

Store at 2 and 8°C for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB1911:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wing S To et al.
The Journal of biological chemistry, 286(17), 14881-14891 (2011-02-18)
Interactions between fibronectin and tenascin-C within the extracellular matrix provide specific environmental cues that dictate tissue structure and cell function. The major binding site for fibronectin lies within the fibronectin type III-like repeats (TNfn) of tenascin-C. Here, we systematically screened

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