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Safety Information

MAB1061

Sigma-Aldrich

Anti-Bone Sialoprotein II Antibody, CT, clone ID1.2

clone ID1.2, Chemicon®, from mouse

Synonym(s):

BSPII, Integrin-binding Sialoprotein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ID1.2, monoclonal

species reactivity

human

should not react with

bovine, rat, pig

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_004967.2

UniProt accession no.

P21815

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IBSP(3381)
pig ... Ibsp(397137)
rat ... Ibsp(24477)

General description

Bone Sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone Sialoprotein is discussed as a potential serum marker for monitoring bone remodeling.

Specificity

Recombinant and native human bone sialoprotein II (BSPII). Does not react with osteopontin (BSPI) or osteonectin.

Immunogen

Bone Sialoprotein isolated from human bones.
Epitope: C-terminus

Application

Research Category
Cell Structure
Research Sub Category
Osteobiology
This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II.
Western blot: (1μg/mL)

Immunohistochemistry on paraffin embedded tissue sections: (1μg/mL)

Immunocytochemistry: (1:100)

ELISA: (1μg/mL)

RIA

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: AB1855

Physical form

Format: Purified
Purified immunoglobulin from culture supernatant. Lyophilized from 50 mM TRIS, pH7.4.

Storage and Stability

Maintain lyophilized material at 2-8°C. Reconstitute with 100μL sterile distilled water and store at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB1061:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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