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Key Documents

Safety Information

MAB1021

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

clone 2C8, Chemicon®, from mouse

Synonym(s):

TNF-alpha

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

clone

2C8, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
neutralization: suitable

isotype

IgG1

NCBI accession no.

NM_000594.2

UniProt accession no.

P01375

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TNF(7124)

Specificity

Human tumor necrosis factor-a (TNF-a)
No cross-reactivity observed with rat neuronal tissue.

Immunogen

Human recombinant tumor necrosis factor α.

Application

Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
Detection of TNF-α in two-site immunoassay.

Immunohistochemistry detected TNF-α.

TNF-α Neutralization Assay

1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.

2. Incubate overnight at 37°C in humidified 5% CO2 incubator.

3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL

4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.

5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.

6. Incubate 24 hours at 37°C, 5% CO2.

7. Score wells for cytotoxicity.

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: 04-1114

Physical form

Format: Purified

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to one year.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB1021-KC:
MAB1021:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Propionibacterium acnes stimulates pro-matrix metalloproteinase-2 expression through tumor necrosis factor-alpha in human dermal fibroblasts.
Jee-Young Choi,Mei Shan Piao,Jee-Bum Lee,Jong Seok Oh,In-Gyu Kim,Seung-Chul Lee
The Journal of Investigative Dermatology null
A Klegeris et al.
Neuroscience letters, 313(1-2), 41-44 (2001-10-31)
We measured the secretion of interleukin (IL)1beta, IL-6 and tumor necrosis factor-alpha (TNF-alpha) from human monocytic (THP-1), astrocytic (U-373 MG) and neuronal (SH-SY5Y) cell lines alone and in co-culture, with and without stimulation by a combination of lipopolysaccharide (LPS) plus
Zifeng Liu et al.
Journal of diabetes, 8(3), 324-335 (2015-04-09)
Resveratrol is a phytoalexin with beneficial effects on human health. The aim of the present study was to investigate the effects of resveratrol on endothelial dysfunction involved in insulin signaling and inflammation. Endothelial cells were stimulated with palmitate (PA) to

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