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I3382

Sigma-Aldrich

Anti-Human IgG (γ-chain specific) antibody produced in goat

affinity isolated antibody, lyophilized powder

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About This Item

MDL number:
MFCD00162442
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

technique(s)

Ouchterlony double diffusion: suitable

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Human IgG (γ-chain specific) antibody is specific for γ chain of all human IgG subclasses.
Immunoglobulins (Igs) belong to the immunoglobulin super-family and have two heavy (H) and two light (L) chains, held together by disulphide linkages. The L chain comprises of one variable N-terminal region and a constant C-terminal region. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4. The IgG heavy chain region is mapped to human chromosome 14.

Immunogen

Purified human IgG

Application

Anti-Human IgG (γ-chain specific) antibody was used as a standard in ELISA and radioimmunoassay.

Biochem/physiol Actions

IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. Digestion of IgG by papain results in generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

I3382-1MG-PW:
I3382-VAR-PW:
I3382-1MG:
I3382PROC:
I3382-VAR:
I3382-2MG:
I3382-BULK-N:
I3382-5X1MG:
I3382-PH:
I3382-BULK:
I3382-BULK-V:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Karl-Heinz Heider et al.
Blood, 118(15), 4159-4168 (2011-07-29)
The tetraspanin CD37 is widely expressed in B-cell malignancies and represents an attractive target for immunotherapy with mAbs. We have chimerized a high-affinity mouse Ab to CD37 and engineered the CH2 domain for improved binding to human Fcγ receptors. The
Wolfgang Sommeregger et al.
Biotechnology and bioengineering, 113(9), 1902-1912 (2016-02-26)
Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the production of biopharmaceuticals. To overcome unfavorable features of CHO cells, a lot of effort is put into cell engineering to improve phenotype. "Omics" studies investigating elevated
R J Cox et al.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 107(3), 289-296 (1999-05-01)
The radio-immunoblot (RIB) assay was used to examine the antibody response to proteins of the vaccine strains induced after influenza vaccination. Vaccination stimulated an antibody response to the surface glycoproteins (HA and NA) and to the internal antigens (NP and
Andreas Maccani et al.
Applied microbiology and biotechnology, 98(17), 7535-7548 (2014-07-24)
MicroRNAs are short non-coding RNAs that play an important role in the regulation of gene expression. Hence, microRNAs are considered as potential targets for engineering of Chinese hamster ovary (CHO) cells to improve recombinant protein production. Here, we analyzed and
V L Esnault et al.
Immunology, 74(4), 714-718 (1991-12-01)
Circulating IgG autoantibodies to myeloperoxidase (MPO) are associated with renal vasculitis and have been implicated in its pathogenesis. However, raised levels of these autoantibodies may persist during clinical remission. We tested whether this paradox could be explained by immunoglobulin subclass
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