HTS090RTA

Ready-to-Assay OT Oxytocin Receptor Frozen Cells

Human

• Synonym(s): Oxytocin-expressing cells
• UNSPSC Code: 41106514
• eCl@ss: 32011203
• NACRES: NA.81
• biological source: human
• manufacturer/tradename: Ready-to-Assay

Properties

• Product Name: Ready-to-Assay OT Oxytocin Receptor Frozen Cells, Human OT GPCR frozen cells for Calcium Flux FLIPR Assays.
• biological source: human
• Quality Level: 100
• manufacturer/tradename: Ready-to-Assay
• technique(s): calcium flux assay: suitable
• NCBI accession no.: NM_000916
• detection method: fluorometric
• shipped in: dry ice

Description

General description

Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Oxytocin is a cyclic 9 amino acid peptide that differs from vasopressin in 2 amino acids. Despite the close similarities in sequence, oxytocin and vasopressin have different biological activities and bind to distinct G protein-coupled receptors. The oxytocin receptor, OT, couples primarily to Gq/11 to mobilize intracellular calcium. In female reproduction, oxytocin promotes uterine contraction and lactation; oxytocin is the most commonly used drug for induction of labor, whereas an oxytocin antagonist, atosiban, is under investigation to suppress preterm labor. Oxytocin/OT interaction in the CNS also plays an important role in stress, male and female sexual response, and sociality (Gimpl and Fahrenholz, 2001). Millipore’s cloned human OT-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OT expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at OT.

Cell Line Description

GPCR Cell Lines

Host cells: Chem-1

Biochem/physiol Actions

GPCR Class: A

Protein Target: OT

Target Sub-Family: Oxytocin

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.

Fifty (50) mL of Media Component.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable