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Safety Information

GF446-M

Sigma-Aldrich

HumanKine® Thermostable bFGF,
Human Recombinant

Synonym(s):

FGF2, prostatropin, Prostatropin, HBGF-2, BFGF

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

Assay

>95% (SDS-PAGE)

Quality Level

100

impurities

<0.5 μg/mg protein endotoxin (<5 EU/μg)

UniProt accession no.

P09038

General description

Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
The HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.

Application

HumanKine products are Xeno-Free
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors

Quality

Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. EC50 is typically ≤0.5 ng/mL

Physical form

Lyophilized from a 0.2 μm filtered solution of 1X PBS.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

GF446-MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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