GF003

Fibroblast Growth Factor basic, human

>95% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture

• Synonym(s): FGF-2, Heparin-Binding Growth Factor 2, bFGF, beta-FGF
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Properties

• product name: Fibroblast Growth Factor basic Protein, Human recombinant,
• biological source: human
• Quality Level: 100
• Assay: >95% (SDS-PAGE)
• manufacturer/tradename: Chemicon^®
• technique(s): cell culture | stem cell: suitable
• impurities: <0.1 EU/μg Endotoxin level (of FGF-b)
• input: sample type neural stem cell(s); sample type mesenchymal stem cell(s); sample type epithelial cells
• NCBI accession no.: NM_002006.4
• UniProt accession no.: P09038

Description

General description

Fibroblast Growth Factor-basic (FGF-b) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo. Human FGF-b is a 17.2 kDa protein containing 155 amino acid residues.

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003-AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.

expressed in E. coli

Application

For most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/mL, depending on the method of culture.

Optimal working dilutions must be determined by end user.

The Fibroblast Growth Factor-basic (FGF-b) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.

Linkage

FA009-1

Physical form

Lyophilized from a 0.7 mg/mL solution in 5mM Tris, pH 7.6 with 150 mM NaCl.

Storage and Stability

Maintain the lyophilized material at -20°C until expiration date as stated on the label.


General applications:

After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted b

Analysis Note

Chemicon′s Human FGF-b is fully biologically active when compared to standards. The ED₅₀ was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable