ABS245

Anti-PKM2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Embryonic enzyme pyruvate kinase M2, Pyruvate kinase isozymes M1/M2, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: monkey, human, mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology), bovine (based on 100% sequence homology), horse (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_872270
• UniProt accession no.: P14618
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PKM(5315)

Description

General description

Pyruvate kinase isozymes M1/M2 (PKM2) is also called Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1 (THBP1) and p58. PKM2 is a glycolytic enzyme that assists in generating ATP. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. This control of glycolysis and is important for tumor cell proliferation and survival. PKM2 is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, and cancer cells.

Specificity

This antibody recognizes PKM2 near the C-terminus.

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human PKM2 near the C-terminus.

Application

Anti-PKM2 Antibody is a highly specific rabbit polyclonal antibody, that targets Pyruvate Kinase & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected PKM2 in HeLa cells.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

Quality

Evaluated by Western Blotting in P19 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PKM2 in 10 µg of P19 cell lysate.

Target description

~60 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
P19 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable